[2018] Identification and Crystallographic Analysis of a New Carbohydrate Acetylesterase (SmAcE1) from Sinorhizobium meliloti
journal : Crystals, 2018
content
ABSTRACT: Carbohydrate-active enzymes (CAZymes) regulate the synthesis, degradation, and modification of the poly—and oligosaccharides in all three kingdoms of life. A novel carbohydrate acetylesterase from Sinorhizobium meliloti, designated SmAcE1, was identified, characterized, and crystallized. This SmAcE1 is classified into the carbohydrate esterase family 3 (CE3) based on the sequence alignments with other currently known carbohydrate esterase (CE) family enzymes. The SmAcE1 was crystallized as a hexamer in a space group P212121 with the unit cell parameters: a = 99.12 Å, b = 148.88 Å, c = 149.84 Å, and α = β = γ = 90.00°. The diffraction data set was collected up to a 2.05 Å resolution. Hydrolysis activity of SmAcE1 towards glucose pentaacetate and cellulose acetate was further confirmed using acetic acid release assay. Further crystallographic and functional analyses studies on SmAcE1 would be followed to fully understand the reaction mechanisms of CEs.
KEYWORDS: carbohydrate acetylesterase, crystal structure, Sinorhizobium meliloti
Author : Changsuk Oh, Bum Han Ryu, Wanki Yoo, Duy Duc Nguyen, Truc Kim, Sung-Chul Ha, T. Doohun Kim, and Kyeong Kyu Kim*
Journal : Crystals, 2018
Publication Date : 2018.1.1
This publication is from
Prof. Doohun
Kim’s
lab